985 The Temporal Changes in the Urine of Severe Traumatic Brain Injury Patients

Abstract Aim Neuroinflammation is a secondary injury mechanism that contributes to significant mortality and morbidity with severe traumatic brain (TBI). This study aimed evaluate the temporal neuroinflammatory pattern of ionised calcium-binding adaptor molecule 1 (Iba1) transmembrane Protein 119 (TMEM119) microglia marker proteins in urine TBI patients for 7 days post-injury. Method Urine samples were acquired from fifteen upon hospital admission subsequent Western blot analysis was used quantify urinary levels Iba-1 TMEM119 expression each sample. The stratified by functional outcomes (group 1, death- vegetative state; group 2, moderate-to- disability; 3, good outcomes). Results Iba1 detected all (100%) whilst 65% samples. mean total normalised increased 2 opposite response observed groups 3. decreased 3 2. However, these findings not statistically significant. No differences found or between favourable unfavourable outcome groups. Conclusions Overall, this cannot associate outcomes. presence patterns markers suggest it may be reflective underlying process merits further studies.